Tris buffer pH 9 (1X)

Catalog Number 12-310005

Alternate Names

    •  TRIS

    •  Tris-buffer

    •  tris(hydroxymethyl)aminomethane

    •  trisaminomethane

    •  2-Amino-2-(hydroxymethyl)propane-1,3-diol

    •  THAM

    •  Trisamine

Suitable for IHC-P

Usage statement RUO

Concentration Ready to use, 1x (RTU)

pH 9

Storage conditions 2-8 °C

Description

Protein cross-links are formed in tissues when liquids containing formalin are used as fixatives. These bonds can mask regions of the antigen, which leads to weak or false-negative staining during the IHC reaction. Tris buffer solution is designed to break protein cross-links and, accordingly, unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue samples. Thus, unmasking allows for a more intense IHC staining.

Packaging 1000 ml