Tris buffer pH 9 (1X)
Catalog Number 12-310005
Alternate Names
• TRIS
• Tris-buffer
• tris(hydroxymethyl)aminomethane
• trisaminomethane
• 2-Amino-2-(hydroxymethyl)propane-1,3-diol
• THAM
• Trisamine
Suitable for IHC-P
Usage statement RUO
Concentration Ready to use, 1x (RTU)
pH 9
Storage conditions 2-8 °C
Description
Protein cross-links are formed in tissues when liquids containing formalin are used as fixatives. These bonds can mask regions of the antigen, which leads to weak or false-negative staining during the IHC reaction. Tris buffer solution is designed to break protein cross-links and, accordingly, unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue samples. Thus, unmasking allows for a more intense IHC staining.
Packaging 1000 ml