Tris-EDTA buffer pH 9 (10X)

Catalog Number 12-310008

Alternate Names

    •  TE buffer

    •  Tris-EDTA buffer

Suitable for IHC-P

Usage statement RUO

Concentration 10x

pH 9

Storage conditions 2-8 °C

Description

This reagent is intended for temperature unmasking of tissue samples fixed in formalin and embedded in paraffin prior to immunohistochemical (IHC) staining. Brief description When liquids containing formalin are used as fixatives, protein cross-links are formed in tissues. These bonds can mask regions of the antigen, which leads to weak or false-negative staining during the IHC reaction. Tris EDTA buffer solution is designed to break protein cross-links and, accordingly, unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue samples. Thus, unmasking allows for a more intense IHC staining.