Universal two-component detection system PrimeVision,

anti-IgG mouse/rabbit, horseradish peroxidase (HRP) and DAB substrate

Catalog Number 78-310004

Suitable for IHC-P

Species Reactivity Mouse and rabbit primary antibodies

Usage Statement RUO (Research use only)

Storage conditions 2-8 °C in the dark, don't freeze

Description

The PrimeVision Universal Two-Piece Detection System is a turnkey system for optimal staining of antigen-antibody complexes. The detection of antigen-antibody complexes is provided by binding to a polyconjugate of a secondary antibody (goat) and polymeric horseradish peroxidase (HRP). The polymer complex (polyconjugate) is visualized with a substrate (3,3'-diaminobenzidine, DAB) forming a colored product. The PrimeVision Detection System detects both mouse and rabbit primary antibodies bound to antigen on tissue sections. The use of primary antibodies manufactured by PrimeBioMed LLC is recommended.

Components

    1. Linker solution (ready-to-use / 1x concentration) - A solution that binds the polymer complex and the antigen-antibody complex.

    2. HRP polyconjugate solution (ready to use / 1x concentration) - Solution containing goat anti-mouse / rabbit antibodies and horseradish peroxidase conjugates.

    3. Solution A: H2O2 buffer (ready to use / 1x concentration) - Used to dilute the DAB substrate chromogen (Solution B).

    4. Solution B: DAB Chromogen Concentrate (see instructions for use for dilution guidance) - Used to visualize polymer complexes.

Packaging

    •  15 ml (~150 tests)

    •  55 ml (~550 tests)

    •  110 ml (~1100 tests)

Application

The recommended protocol for use in immunohistochemistry studies is given in the instructions.

References

    1. Shan-Rong Shi, James Guo, Richard J.cote, Lillian Young, Debra Hawes, Yan Shi, Sandra Thu and Clive R. Taylor, Applied Immunohistochemistry & Molecular Morphology, vol 7,201-208,1999